Blar i forfatter "Michetti, Davide"

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    • A comparative study of cold-and warmadapted Endonucleases A using sequence analyses and molecular Dynamics simulations 

      Michetti, Davide; Brandsdal, Bjørn Olav; Bon, Davide; Isaksen, Geir Villy; Tiberti, Matteo; Papaleo, Elena (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-02-13)
      The psychrophilic and mesophilic endonucleases A (EndA) from Aliivibrio salmonicida (VsEndA) and Vibrio cholera (VcEndA) have been studied experimentally in terms of the biophysical properties related to thermal adaptation. The analyses of their static X-ray structures was no sufficient to rationalize the determinants of their adaptive traits at the molecular level. Thus, we used Molecular Dynamics ...

    • Surface properties tune thermal adaptation of enzymes - Computational studies of endonuclease A 

      Michetti, Davide (Doctoral thesis; Doktorgradsavhandling, 2017-04-26)
      The endonucleases A from Aliivibrio salmonicida (VsEndA, cold-adapted) and Vibrio cholerae (VcEndA, warm-adapted) have been studied in this work. The goal was to compare these two systems, in order to find structural differences that could explain the mechanism of cold adaptation in the psychrophilic EndA. These two enzymes have previously been studied experimentally and the crystal structures have ...